Shu-Fung Lo

Assistant of Research of

Department of Agronomy

Chia-Yi Agricultural Experiment Station

Taiwan Agricultural Research Institute

Phone886-5-2771341

Fax886-5-2773630

Email :losf@dns.caes.gov.tw

Education

B. S.(1986)in Department of Agronomy, National Chung-Hsing University

M. S.(1995) in Department of Agronomy, National Chung-Hsing University

Area of Research Interest

The research of tissue culture of plant

Publication

Journal paper

1. Lo, S. F. and C. H. Liao 1993. Studies on the In vitro maintenance techniques of sweet potato (Ipomoea batata L.) I. Influence of carbon source. Jour. Agric. Res. China 42(1)30-36.

Experiments were conducted to study the influence of sugar sources on the in vitro growth and maintenance of sweet potato cuttings for the purpose of efficient germplasm preservation. Stem segments of virus-free sweet potato plants were successfully cultured on a medium containing Murashige and Skoog(MS)inorganic salts supplemented with 0.4mg/l thiamine-HCL, 100mg/l myo-inositol, 1mg/l IAA, 6% sucrose and 0.8% Difco agar. In addition to active growth of the explants in the test tube, the survival rate and growth vigor of the cuttings after transplanting to pots were also high as compared to other treatments. In order to prolong the time interval between subcultures of the in vitro preserved sweet potato stem segments, the addition of 3% glucose as the sole carbon source to the medium was effective to lower the growth rate of the explants. The interval between successive subcultures could be extended to as long as 6 months. The growth vigor could be resumed after transfer the explants to culture medium containing 3% sucrose.

2. Lo, S. F. and H. S. Tsay 1996. In vitro plant regeneration from cultured leaves of sweet potato. Jour. Agric. Res. China 45(3)241-249.

The ability of roots formation and plant regeneration from leaf was higher than that of petiole in different sweet potato (Ipomoea batatas L.) varieties when the explants were cultured on a Murashige and Skoog (1962) (MS) medium supplement with 3﹪sucrose and 0.9﹪agar. The frequency of callus induction was as high as 100﹪when leaf or petiole was cultured on the MS medium containing 0.2∼1.0mg/l thidiazuron (TDZ). However, TDZ has no effect on root formation and plant regeneration. The basal part of 30 day-old leaves dissected from plants derived from node culture was found to be a good source for both organ regeneration and callus formation. Using leaves cultured on a medium containing MS salts and 0.1∼0.5mg/l NAA more than 85﹪root formation and 92﹪callus induction rates were recorded. As high as 78﹪plant regeneration rate was observed when leaves were cultured on a MS medium containing 0.1mg/l NAA.